eDNA Biodiversity Detection

AUS-AID-CON-DNA General Moderate confidence

Benchmark Value

50000 read pairs per sample
Direction: Higher is desirable ↑
Form: Point

Evidence & Context

a read depth of 50,000 read pairs per sample has been recommended in some fish eDNA studies to adequately capture diversity measures.

Metric Definition:

Sequencing depth required to adequately capture diversity measures in fish eDNA studies

Benchmark Definition:

This benchmark represents the recommended sequencing depth of 50,000 read pairs per sample to adequately capture biodiversity diversity measures in fish eDNA studies within the Arid Interior Dunefields & Sandy Deserts biome under conservation land use.

Justification:

Recommended depth to ensure diversity measures are adequately captured.

Sources (1)

Preview of Turning to eDNA to track endangered species in East Australia - Illumina, accessed July 11, 2025,
Turning to eDNA to track endangered species in East Australia - Illumina, accessed July 11, 2025, GreyLiterature

A comprehensive evaluation of taxonomic classifiers in marine ...

View Source

Supporting Sources (38)

Additional references from the underlying research that informed this benchmark.

Preview of (PDF) Australia's marine fishes DNA barcode reference library for ..., accessed on May 23, 2025
(PDF) Australia's marine fishes DNA barcode reference library for ..., accessed on May 23, 2025
Methodology Source GreyLiterature

(PDF) Australia's marine fishes DNA barcode reference library for ...

View Source
Preview of A Confidence Scoring Procedure for eDNA Metabarcoding Records and Its Application to a Global Marine Fish Dataset - Archimer, accessed August 4, 2025
A Confidence Scoring Procedure for eDNA Metabarcoding Records and Its Application to a Global Marine Fish Dataset - Archimer, accessed August 4, 2025
Contextual Support Journal

Towards the standardisation of bioinformatic eDNA workflows for ocean conservation: Considerations and best practice for robust amplicon data analysis

View Source
Preview of A Confidence Scoring Procedure for eDNA Metabarcoding Records and Its Application to a Global Marine Fish Dataset - ResearchGate, accessed on May 23, 2025
A Confidence Scoring Procedure for eDNA Metabarcoding Records and Its Application to a Global Marine Fish Dataset - ResearchGate, accessed on May 23, 2025
Methodology Source Journal

A Confidence Scoring Procedure for eDNA Metabarcoding Records and Its Application to a Global Marine Fish Dataset - ResearchGate

View Source
Preview of A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository
A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository
Contextual Support Journal

TICI: a taxon-independent community index for eDNA-based ecological health assessment

View Source
Preview of Application of environmental DNA (eDNA) and eRNA technology for pest and disease surveillance, accessed on May 23, 2025
Application of environmental DNA (eDNA) and eRNA technology for pest and disease surveillance, accessed on May 23, 2025
Methodology Source GreyLiterature

Application of environmental DNA (eDNA) and eRNA technology for pest and disease surveillance

View Source
Preview of Best practice guidelines for environmental DNA biomonitoring in Australia and New Zealand - ResearchOnline@JCU
Best practice guidelines for environmental DNA biomonitoring in Australia and New Zealand - ResearchOnline@JCU
Contextual Support Journal

Using low volume eDNA methods to sample pelagic marine animal ..., accessed on May 23, 2025, researchonline.jcu.edu.au

View Source
Preview of Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis - Frontiers, accessed August 10, 2025
Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis - Frontiers, accessed August 10, 2025
Contextual Support Journal

Beyond Detection – The Future of eDNA Research (Post-Event Summary)

View Source
Preview of Community baseline vertebrate biodiversity surveys in the Hornsby ..., accessed July 24, 2025
Community baseline vertebrate biodiversity surveys in the Hornsby ..., accessed July 24, 2025
Contextual Support

Past, present, and future perspectives on environmental DNA (eDNA) metabarcoding - ScholarWorks @ UTRGV

View Source
Preview of eDNA biodiversity from space: predicting soil bacteria and fungi ..., accessed on May 23, 2025
eDNA biodiversity from space: predicting soil bacteria and fungi ..., accessed on May 23, 2025
Contextual Support GreyLiterature

eDNA biodiversity from space: predicting soil bacteria and fungi ...

View Source
Preview of eDNA Expeditions eDNA sampling protocol - UNESCO, accessed on May 23, 2025
eDNA Expeditions eDNA sampling protocol - UNESCO, accessed on May 23, 2025
Methodology Source Journal

Methods to maximise recovery of environmental DNA from water ...

View Source
Preview of eDNA explained: unlocking nature's hidden biodiversity - CSIRO, accessed August 12, 2025
eDNA explained: unlocking nature's hidden biodiversity - CSIRO, accessed August 12, 2025
Contextual Support Government

www.csiro.au

View Source
Preview of eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions
eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions
Contextual Support Journal

A manager's guide to using eDNA metabarcoding in marine ecosystems - PeerJ

View Source
Preview of eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed July 22, 2025,
eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed July 22, 2025,
Methodology Source Government

Integrating environmental DNA science into Australia's marine parks: a roadmap - CSIRO Research Publications Repository

View Source
Preview of Environment Monitoring Systems & Data for Ecosystem Studies ..., accessed July 30, 2025,
Environment Monitoring Systems & Data for Ecosystem Studies ..., accessed July 30, 2025,
Methodology Source Government

(PDF) Evaluation of genetic markers for the metabarcoding of ...

View Source
Preview of Environmental DNA - resource | IUCN, accessed on May 23, 2025
Environmental DNA - resource | IUCN, accessed on May 23, 2025
Contextual Support GreyLiterature

Environmental DNA - resource | IUCN

View Source
Preview of Environmental DNA (eDNA) - Australian Platypus Conservancy
Environmental DNA (eDNA) - Australian Platypus Conservancy
Contextual Support GreyLiterature

Effect of eDNA metabarcoding temporal sampling ... - Frontiers

View Source
Preview of Environmental DNA (eDNA) - U.S. Fish and Wildlife Service, accessed August 12, 2025
Environmental DNA (eDNA) - U.S. Fish and Wildlife Service, accessed August 12, 2025
Contextual Support Journal

Joint media release: eDNA results reveal rich biodiversity in the Murray–Darling Basin

View Source
Preview of Environmental DNA in practice: a roadmap towards integrating eDNA methods in marine monitoring programs, accessed on May 23, 2025
Environmental DNA in practice: a roadmap towards integrating eDNA methods in marine monitoring programs, accessed on May 23, 2025
Methodology Source GreyLiterature

Environmental DNA in practice: a roadmap towards integrating eDNA methods in marine monitoring programs

View Source
Preview of Environmental DNA metabarcoding describes biodiversity across marine gradients - Oxford Academic, accessed August 4, 2025
Environmental DNA metabarcoding describes biodiversity across marine gradients - Oxford Academic, accessed August 4, 2025
Contextual Support Journal

Environmental DNA metabarcoding describes biodiversity across marine gradients - Oxford Academic

View Source
Preview of Harnessing the Power of eDNA for Biodiversity Monitoring, accessed on May 23, 2025
Harnessing the Power of eDNA for Biodiversity Monitoring, accessed on May 23, 2025
Methodology Source Journal

Harnessing the Power of eDNA for Biodiversity Monitoring

View Source
Preview of Insights into the representativeness of biodiversity assessment in large reservoir through eDNA metabarcoding | PLOS One, accessed on May 23, 2025
Insights into the representativeness of biodiversity assessment in large reservoir through eDNA metabarcoding | PLOS One, accessed on May 23, 2025
Methodology Source Journal

Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis - Frontiers

View Source
Preview of Inter-laboratory ring test for environmental DNA extraction protocols: implications for marine megafauna detection using three novel qPCR assays - Metabarcoding and Metagenomics, accessed on May 23, 2025
Inter-laboratory ring test for environmental DNA extraction protocols: implications for marine megafauna detection using three novel qPCR assays - Metabarcoding and Metagenomics, accessed on May 23, 2025
Methodology Source Journal

Inter-laboratory ring test for environmental DNA extraction protocols: implications for marine megafauna detection using three novel qPCR assays - Metabarcoding and Metagenomics

View Source
Preview of Joint media release: eDNA results reveal rich biodiversity in the ..., accessed August 10, 2025
Joint media release: eDNA results reveal rich biodiversity in the ..., accessed August 10, 2025
Contextual Support Government

Murray–Darling Basin Authority. (2024). eDNA results reveal rich biodiversity in the Murray–Darling Basin. Joint Media Release.

View Source
Preview of NAMERS: a purpose-built reference DNA sequence database to support applied eDNA metabarcoding, accessed on May 23, 2025
NAMERS: a purpose-built reference DNA sequence database to support applied eDNA metabarcoding, accessed on May 23, 2025
Methodology Source GreyLiterature

NAMERS: a purpose-built reference DNA sequence database to support applied eDNA metabarcoding

View Source
Preview of National Ecosystem Accounts, experimental estimates methodology ..., accessed on May 23, 2025
National Ecosystem Accounts, experimental estimates methodology ..., accessed on May 23, 2025
Methodology Source Government

National Ecosystem Accounts, experimental estimates methodology ...

View Source
Preview of National eDNA Reference Centre - EcoDNA
National eDNA Reference Centre - EcoDNA
Regulatory Framework Government

National eDNA Reference Centre - EcoDNA

View Source
Preview of Perfect match: DNA library to help identify species - CSIRO
Perfect match: DNA library to help identify species - CSIRO
Contextual Support Government

What lies beneath the water? | Murray–Darling Basin Authority

View Source
Preview of Quantitative environmental DNA metabarcoding shows high ..., accessed on May 23, 2025
Quantitative environmental DNA metabarcoding shows high ..., accessed on May 23, 2025
Methodology Source Journal

Quantitative environmental DNA metabarcoding shows high ...

View Source
Preview of Revolutionising environmental monitoring using eDNA - CSIRO
Revolutionising environmental monitoring using eDNA - CSIRO
Contextual Support Government

Perfect match: DNA library to help identify species - CSIRO

View Source
Preview of Revolutionising environmental monitoring using eDNA - CSIRO, accessed August 12, 2025
Revolutionising environmental monitoring using eDNA - CSIRO, accessed August 12, 2025
Contextual Support Government

Environmental DNA (eDNA) - U.S. Fish and Wildlife Service

View Source
Preview of Soil microbiome and biodiversity implications – are we ignoring cost-effective proxies of biodiversity measures? | TERN Australia, accessed on June 7, 2025,
Soil microbiome and biodiversity implications – are we ignoring cost-effective proxies of biodiversity measures? | TERN Australia, accessed on June 7, 2025,
Contextual Support GreyLiterature

Environment Monitoring Systems & Data for Ecosystem Studies ...

View Source
Preview of System of Environmental-Economic Accounting (SEEA), accessed on May 23, 2025
System of Environmental-Economic Accounting (SEEA), accessed on May 23, 2025
Methodology Source Government

System of Environmental-Economic Accounting (SEEA)

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Preview of Understanding Natural Capital And The Accounting For Nature Framework - Anthesis Group, accessed on May 23, 2025
Understanding Natural Capital And The Accounting For Nature Framework - Anthesis Group, accessed on May 23, 2025
Methodology Source GreyLiterature

Understanding Natural Capital And The Accounting For Nature Framework - Anthesis Group

View Source
Preview of Using low volume eDNA methods to sample pelagic marine animal ...
Using low volume eDNA methods to sample pelagic marine animal ...
Contextual Support Journal

Using low volume eDNA methods to sample pelagic marine animal ...

View Source
Preview of What are the best practices for curating eDNA custom barcode ..., accessed on May 23, 2025
What are the best practices for curating eDNA custom barcode ..., accessed on May 23, 2025
Methodology Source Journal

What are the best practices for curating eDNA custom barcode ...

View Source
Preview of What is eDNA method standardisation and why do we need it?, accessed on May 23, 2025
What is eDNA method standardisation and why do we need it?, accessed on May 23, 2025
Methodology Source GreyLiterature

What is eDNA method standardisation and why do we need it?

View Source
Preview of What lies beneath the water? | Murray–Darling Basin Authority, accessed August 12, 2025
What lies beneath the water? | Murray–Darling Basin Authority, accessed August 12, 2025
Regulatory Framework Government

Accounting for Nature and measuring nature repair - Greening ...

View Source
Preview of www.csiro.au, accessed on May 23, 2025
www.csiro.au, accessed on May 23, 2025
Contextual Support Government

www.csiro.au, accessed on May 23, 2025

View Source

Context

  • Region Australia
  • Biome Arid Interior Dunefields & Sandy Deserts
  • Land Use Conservation / Protected Natural Areas
  • Assessment Not Stated
  • Evidence Type ReferenceCondition

Lifecycle

  • Status Retired
  • Version 1
  • Effective From 9 Jun 2026
  • Effective To 9 Jun 2026

Notes

Specific to fish eDNA studies. AssessmentContext defaulted to 'Not Stated' because the source document did not state one.

Related Benchmarks

Other benchmarks in the AUS-AID-CON-DNA family.

50000 read pairs per sample Moderate
Conservation / Protected Natural Areas · Not Stated
Point Active v1
50000 read pairs per sample Moderate
Conservation / Protected Natural Areas · Conservation Target
Point Retired v1
50000 read pairs per sample Moderate
Conservation / Protected Natural Areas · Conservation Target
Point Retired v1
50000 read pairs per sample Moderate
Conservation / Protected Natural Areas · Not Stated
Point Retired v1