Microbial Respiration

AUS-TGP-AGR-SMR General Moderate confidence

Benchmark Value

9.6 mg CO2-C/kg/day

Direction: Higher is desirable ↑

Form: Point

Evidence & Context

Thus, the derived benchmark for microbial respiration representing a best available condition in a temperate Australian regenerative cropping system is approximately 9.6 mg CO2-C/kg/day.

Metric Definition:

Basal microbial respiration, the flux of carbon dioxide (CO2) from the soil, specifically quantifying the metabolic activity of the heterotrophic microbial community under standardized laboratory conditions.

Benchmark Definition:

Basal microbial respiration is the rate of CO2 flux from soil microbial metabolism under controlled conditions, indicating soil biological activity in temperate Australian agricultural cropping systems.

Justification:

The benchmark is derived from a microbial biomass carbon (MBC) value of ~400 mg C/kg soil from well-managed southern Australian cropping systems combined with an inferred metabolic quotient (qCO2) of 1.0 g CO2-C/mg MBC/h representing a highly efficient microbial community.

Supporting Sources (49)

Additional references from the underlying research that informed this benchmark.

Preview of A review on effective soil health bio-indicators for ecosystem restoration and sustainability, accessed August 5, 2025,

A review on effective soil health bio-indicators for ecosystem restoration and sustainability, accessed August 5, 2025,

Contextual Support Journal

Seasonal variations in soil characteristics control microbial respiration and carbon use under tree plantations in the middle gangetic region - PMC - PubMed Central, accessed July 28, 2025

Preview of Basal and specific microbial respiration in semiarid agricultural soils: Organic amendment and irrigation management effects - Taylor & Francis Online, accessed August 5, 2025,

Basal and specific microbial respiration in semiarid agricultural soils: Organic amendment and irrigation management effects - Taylor & Francis Online, accessed August 5, 2025,

Contextual Support Direct Evidence Journal

Basal and specific microbial respiration in semiarid agricultural soils: Organic amendment and irrigation management effects - Taylor & Francis Online

Preview of Bioregions and EVC benchmarks - Environment, accessed August 5, 2025,

Bioregions and EVC benchmarks - Environment, accessed August 5, 2025,

Contextual Support Government

Eastern NSW Plant Community Type Percentage Cleared Calculation Technical Notes - Environment and Heritage

Preview of Changes in Soil Microbial Community Structure Influenced by Agricultural Management Practices in a Mediterranean Agro-Ecosystem | PLOS One - Research journals, accessed August 5, 2025,

Changes in Soil Microbial Community Structure Influenced by Agricultural Management Practices in a Mediterranean Agro-Ecosystem | PLOS One - Research journals, accessed August 5, 2025,

Contextual Support Journal

Changes in Soil Microbial Community Structure Influenced by Agricultural Management Practices in a Mediterranean Agro-Ecosystem | PLOS One - Research journals, accessed July 20, 2025,

Preview of Differential Nutrient Limitation of Soil Microbial Biomass and Metabolic Quotients (qCO2): Is There a Biological Stoichiometry of Soil Microbes? | PLOS One - Research journals, accessed August 5, 2025,

Differential Nutrient Limitation of Soil Microbial Biomass and Metabolic Quotients (qCO2): Is There a Biological Stoichiometry of Soil Microbes? | PLOS One - Research journals, accessed August 5, 2025,

Contextual Support Journal

Differential Nutrient Limitation of Soil Microbial Biomass and Metabolic Quotients (qCO2): Is There a Biological Stoichiometry of Soil Microbes? | PLOS One - Research journals

Preview of Discover the Top 6 Benefits of Regenerative Farming in Australia, accessed August 5, 2025,

Discover the Top 6 Benefits of Regenerative Farming in Australia, accessed August 5, 2025,

Contextual Support GreyLiterature

The relationships between land management practices and soil condition and the quality of ecosystem services delivered from agri - DAFF

Preview of Effects of Semiarid Wheat Agriculture Management Practices on Soil ..., accessed August 5, 2025,

Effects of Semiarid Wheat Agriculture Management Practices on Soil ..., accessed August 5, 2025,

Contextual Support

Effects of Semiarid Wheat Agriculture Management Practices on Soil Microbial Properties: A Review - MDPI, accessed July 23, 2025,

Preview of Fact Sheets Chemical Indicators - Soil Quality, accessed August 5, 2025,

Fact Sheets Chemical Indicators - Soil Quality, accessed August 5, 2025,

Contextual Support GreyLiterature

Phosphorus Forms and Functions - Soil Management, accessed July 22, 2025,

Preview of How to determine an OPTIMAL soil respiration (or microbial respiration)? - ResearchGate, accessed August 5, 2025,

How to determine an OPTIMAL soil respiration (or microbial respiration)? - ResearchGate, accessed August 5, 2025,

Contextual Support GreyLiterature

Soil Health Testing - Agronomic Insights, accessed August 13, 2025,

Preview of Impact of a change in tillage and crop residue management practice ..., accessed August 5, 2025,

Impact of a change in tillage and crop residue management practice ..., accessed August 5, 2025,

Contextual Support

Impact of a change in tillage and crop residue management practice ...

Preview of Indicators for evaluating soil quality - UF/IFAS, accessed August 5, 2025,

Indicators for evaluating soil quality - UF/IFAS, accessed August 5, 2025,

Contextual Support Journal

Soil health: the foundation of sustainable agriculture - 2001 workshop proceedings - NSW Department of Primary Industries, accessed July 19, 2025

Preview of Influence of Agricultural Practices on Bacterial Community of Cultivated Soils - MDPI, accessed August 5, 2025,

Influence of Agricultural Practices on Bacterial Community of Cultivated Soils - MDPI, accessed August 5, 2025,

Contextual Support Journal

Influence of Agricultural Practices on Bacterial Community of Cultivated Soils - MDPI

Preview of Inherent Factors Affecting Soil Respiration - Solvita, accessed August 5, 2025,

Inherent Factors Affecting Soil Respiration - Solvita, accessed August 5, 2025,

Contextual Support Journal

Inherent Factors Affecting Soil Respiration - Solvita

Preview of Inherent Factors Affecting Soil Respiration - USDA, accessed July 30, 2025,

Inherent Factors Affecting Soil Respiration - USDA, accessed July 30, 2025,

Contextual Support Journal

Twenty years of progress, challenges, and opportunities in measuring and understanding soil respiration, accessed August 13, 2025,

Preview of Linking Soil Microbial Activity to Water and Air-Phase Contents and Diffusivities | Request PDF - ResearchGate, accessed August 5, 2025,

Linking Soil Microbial Activity to Water and Air-Phase Contents and Diffusivities | Request PDF - ResearchGate, accessed August 5, 2025,

Contextual Support Direct Evidence

Linking Soil Microbial Activity to Water and Air-Phase Contents and Diffusivities | Request PDF - ResearchGate

$Preview of Long-term land use change in Australia from native forest decreases all fractions of soil organic carbon, including resistant organic carbon, for cropping but not sown pasture - ResearchGate, accessed August 5, 2025,$

Long-term land use change in Australia from native forest decreases all fractions of soil organic carbon, including resistant organic carbon, for cropping but not sown pasture - ResearchGate, accessed August 5, 2025,

Contextual Support Journal

Soil microbial activity and litter turnover in native grazed and ungrazed rangelands in semiarid ecosystem - ResearchGate, accessed July 16, 2025,

Preview of Mapping Australia's soil biodiversity - CSIRO, accessed August 5, 2025,

Mapping Australia's soil biodiversity - CSIRO, accessed August 5, 2025,

Contextual Support GreyLiterature

Mapping Australia's soil biodiversity - CSIRO, accessed August 9, 2025,

Preview of Meta-analysis approach to assess effect of tillage on microbial biomass and enzyme activities - Hoorman Soil Health Services, accessed August 5, 2025,

Meta-analysis approach to assess effect of tillage on microbial biomass and enzyme activities - Hoorman Soil Health Services, accessed August 5, 2025,

Contextual Support Journal

Meta-analysis approach to assess effect of tillage on microbial biomass and enzyme activities - Hoorman Soil Health Services

Preview of Metabolic quotient of the soil microflora in relation to plant succession - PubMed, accessed August 5, 2025,

Metabolic quotient of the soil microflora in relation to plant succession - PubMed, accessed August 5, 2025,

Direct Evidence

The metabolic quotient for CO 2 (qCO 2 ) as a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils. - CABI Digital Library

Preview of Microbial Biomass Carbon - NSW | Fact Sheets | soilquality.org.au, accessed August 5, 2025,

Microbial Biomass Carbon - NSW | Fact Sheets | soilquality.org.au, accessed August 5, 2025,

Contextual Support GreyLiterature

Microbial Biomass | Fact Sheets | soilquality.org.au, accessed July 18, 2025,

Preview of Microbial Metabolic Quotient is a Dynamic Indicator of Soil Health: Trends, Implications and Perspectives (Review) | CoLab, accessed August 5, 2025,

Microbial Metabolic Quotient is a Dynamic Indicator of Soil Health: Trends, Implications and Perspectives (Review) | CoLab, accessed August 5, 2025,

Contextual Support Irrelevant

Microbial Metabolic Quotient is a Dynamic Indicator of Soil Health: Trends, Implications and Perspectives (Review) | CoLab

Preview of Real-Time and Rapid Respiratory Response of the Soil Microbiome to Moisture Shifts - PMC, accessed August 5, 2025,

Real-Time and Rapid Respiratory Response of the Soil Microbiome to Moisture Shifts - PMC, accessed August 5, 2025,

Contextual Support Journal

Real-Time and Rapid Respiratory Response of the Soil Microbiome to Moisture Shifts - PMC

Preview of Regenerative Agriculture - a literature review on the practices and mechanisms used to improve soil health - DPIRD's Digital library, accessed August 5, 2025,

Regenerative Agriculture - a literature review on the practices and mechanisms used to improve soil health - DPIRD's Digital library, accessed August 5, 2025,

Contextual Support

Regenerative Land Management - HEALTHY SOILS AUSTRALIA

Preview of Regenerative agriculture in Australia: the changing face of farming - Frontiers, accessed August 4, 2025

Regenerative agriculture in Australia: the changing face of farming - Frontiers, accessed August 4, 2025

Contextual Support Journal

NCC: Regenerative Agriculture and Soil Health - Wet Tropics Plan, accessed July 27, 2025,

Preview of Regenerative opportunities for building soil biological resilience – a ..., accessed May 11, 2025

Regenerative opportunities for building soil biological resilience – a ..., accessed May 11, 2025

Direct Evidence GreyLiterature

Soil and fertilizer phosphorus: Effects on plant P supply and mycorrhizal development - Canadian Science Publishing, accessed August 28, 2025

Preview of Responses of soil nutrients and microbial activity to the mill-mud application in a compaction-affected sugarcane field - CSIRO Publishing, accessed August 5, 2025,

Responses of soil nutrients and microbial activity to the mill-mud application in a compaction-affected sugarcane field - CSIRO Publishing, accessed August 5, 2025,

Contextual Support Direct Evidence Journal

Responses of soil nutrients and microbial activity to the mill-mud application in a compaction-affected sugarcane field - CSIRO Publishing

Preview of Short-term contributions of cover crop surface residue return to soil carbon and nitrogen contents in temperate Australia - ResearchGate, accessed May 11, 2025

Short-term contributions of cover crop surface residue return to soil carbon and nitrogen contents in temperate Australia - ResearchGate, accessed May 11, 2025

Contextual Support Journal

Short-term contributions of cover crop surface residue return to soil carbon and nitrogen contents in temperate Australia - ResearchGate

Preview of Soil aggregation and associated microbial communities modify the impact of agricultural management on carbon content - PubMed, accessed August 5, 2025,

Soil aggregation and associated microbial communities modify the impact of agricultural management on carbon content - PubMed, accessed August 5, 2025,

Contextual Support Irrelevant Journal

Soil aggregation and associated microbial communities modify the impact of agricultural management on carbon content - PubMed

Preview of Soil Carbon and Respiration – Soils Laboratory Manual - New Prairie Press Open Book Publishing, accessed August 5, 2025,

Soil Carbon and Respiration – Soils Laboratory Manual - New Prairie Press Open Book Publishing, accessed August 5, 2025,

Contextual Support Methodology Source GreyLiterature

Soil Carbon and Respiration – Soils Laboratory Manual - New Prairie Press Open Book Publishing

Preview of Soil condition | NSW State of the Environment, accessed August 10, 2025,

Soil condition | NSW State of the Environment, accessed August 10, 2025,

Contextual Support Government

The relationships between land management practices and soil condition and the quality of ecosystem services delivered from agri - DAFF, accessed July 28, 2025,

Preview of Soil Health Assessment - Natural Resources Conservation Service - USDA, accessed August 5, 2025,

Soil Health Assessment - Natural Resources Conservation Service - USDA, accessed August 5, 2025,

Contextual Support Methodology Source Government

Soil Health Assessment - Natural Resources Conservation Service - USDA

Preview of Soil microbial activity (soil respiration in mg CO 2 / g dry soil - ResearchGate, accessed August 5, 2025,

Soil microbial activity (soil respiration in mg CO 2 / g dry soil - ResearchGate, accessed August 5, 2025,

Contextual Support Methodology Source Journal

Soil microbial activity (soil respiration in mg CO 2 / g dry soil - ResearchGate

Preview of Soil Microbial Biomass and Respiration - microBIOMETER, accessed August 5, 2025,

Soil Microbial Biomass and Respiration - microBIOMETER, accessed August 5, 2025,

Contextual Support GreyLiterature

Soil Microbial Biomass and Respiration - microBIOMETER, accessed on June 5, 2025

Preview of Soil microbial biomass—Interpretation and consideration for soil monitoring - ResearchGate, accessed August 5, 2025,

Soil microbial biomass—Interpretation and consideration for soil monitoring - ResearchGate, accessed August 5, 2025,

Contextual Support Journal

Soil microbial biomass—Interpretation and consideration for soil monitoring - ResearchGate

Preview of Soil Microbial Biomass, Basal Respiration and Enzyme Activity of Main Forest Types in the Qinling Mountains | PLOS One - Research journals, accessed August 5, 2025,

Soil Microbial Biomass, Basal Respiration and Enzyme Activity of Main Forest Types in the Qinling Mountains | PLOS One - Research journals, accessed August 5, 2025,

Contextual Support

Major and persistent shifts in below‐ground ... - yadvinder malhi

Preview of Soil microbial biomass, metabolic quotient, and carbon and nitrogen mineralisation in 25-year-old Pinus radiata agroforestry regimes | Request PDF - ResearchGate, accessed August 5, 2025,

Soil microbial biomass, metabolic quotient, and carbon and nitrogen mineralisation in 25-year-old Pinus radiata agroforestry regimes | Request PDF - ResearchGate, accessed August 5, 2025,

Contextual Support Methodology Source Journal

Soil microbial biomass, metabolic quotient, and carbon and nitrogen mineralisation in 25-year-old Pinus radiata agroforestry regimes | Request PDF - ResearchGate

Preview of Soil microbial carbon use efficiency and biomass turnover in a long-term fertilization experiment in a temperate grassland - AGRIS, accessed August 5, 2025,

Soil microbial carbon use efficiency and biomass turnover in a long-term fertilization experiment in a temperate grassland - AGRIS, accessed August 5, 2025,

Contextual Support Methodology Source Journal

Soil microbial carbon use efficiency and biomass turnover in a long-term fertilization experiment in a temperate grassland - AGRIS

Preview of Soil microbial respiration adapts to ambient temperature in global drylands - PMC, accessed August 5, 2025,

Soil microbial respiration adapts to ambient temperature in global drylands - PMC, accessed August 5, 2025,

Contextual Support

A review on effective soil health bio-indicators for ecosystem restoration and sustainability, accessed July 28, 2025

Preview of Soil Research - CSIRO PUBLISHING, accessed August 5, 2025,

Soil Research - CSIRO PUBLISHING, accessed August 5, 2025,

Contextual Support Direct Evidence Journal

Soil Research - CSIRO PUBLISHING

Preview of Soil Respiration - Natural Resources Conservation Service, accessed July 30, 2025,

Soil Respiration - Natural Resources Conservation Service, accessed July 30, 2025,

Contextual Support Journal

Inherent Factors Affecting Soil Respiration - USDA, accessed August 10, 2025,

Preview of Soil respiration - Wikipedia, accessed July 30, 2025,

Soil respiration - Wikipedia, accessed July 30, 2025,

Contextual Support

Soil respiration - Wikipedia, accessed August 13, 2025,

Preview of Soil respiration rate and its sensitivity to temperature in pasture systems of dry-tropics - Taylor & Francis Online, accessed July 30, 2025,

Soil respiration rate and its sensitivity to temperature in pasture systems of dry-tropics - Taylor & Francis Online, accessed July 30, 2025,

Contextual Support Journal

Soil respiration rate and its sensitivity to temperature in pasture systems of dry-tropics - Taylor & Francis Online, accessed August 13, 2025,

Preview of Soil Science Curriculum - South Dakota Soil Health Coalition, accessed August 5, 2025,

Soil Science Curriculum - South Dakota Soil Health Coalition, accessed August 5, 2025,

Contextual Support Journal

Soil Science Curriculum - South Dakota Soil Health Coalition

Preview of Soil temperature, microbial biomass and enzyme activity are the critical factors affecting soil respiration in different soil layers in Ziwuling Mountains, China - Frontiers, accessed July 30, 2025,

Soil temperature, microbial biomass and enzyme activity are the critical factors affecting soil respiration in different soil layers in Ziwuling Mountains, China - Frontiers, accessed July 30, 2025,

Contextual Support

Soil Respiration - Natural Resources Conservation Service, accessed July 28, 2025,

Preview of Soil temperature, microbial biomass and enzyme activity are the critical factors affecting soil respiration in different soil layers in Ziwuling Mountains, China - PMC, accessed August 5, 2025,

Soil temperature, microbial biomass and enzyme activity are the critical factors affecting soil respiration in different soil layers in Ziwuling Mountains, China - PMC, accessed August 5, 2025,

Contextual Support Journal

Forest Soil Bacteria: Diversity, Involvement in Ecosystem Processes, and Response to Global Change - PubMed Central

Preview of Temperature sensitivity of soil respiration rates enhanced by microbial community response - PubMed, accessed July 30, 2025,

Temperature sensitivity of soil respiration rates enhanced by microbial community response - PubMed, accessed July 30, 2025,

Contextual Support Journal

Temperature sensitivity of soil respiration rates enhanced by microbial community response - PubMed, accessed August 13, 2025,

Preview of The influence of soil types and agricultural management practices on soil chemical properties and microbial dynamics - Frontiers, accessed August 5, 2025,

The influence of soil types and agricultural management practices on soil chemical properties and microbial dynamics - Frontiers, accessed August 5, 2025,

Contextual Support Journal

The influence of soil types and agricultural management practices on soil chemical properties and microbial dynamics - Frontiers

Preview of Tillage practices altered labile soil organic carbon and microbial function without affecting crop yields - CSIRO Publishing, accessed August 5, 2025,

Tillage practices altered labile soil organic carbon and microbial function without affecting crop yields - CSIRO Publishing, accessed August 5, 2025,

Contextual Support Journal

Tillage practices altered labile soil organic carbon and microbial function without affecting crop yields - CSIRO Publishing

Preview of View of Efficacy of the Ecosystem Services Approach in Transitioning to Regenerative Agriculture in Australia | Reinvention, accessed August 5, 2025,

View of Efficacy of the Ecosystem Services Approach in Transitioning to Regenerative Agriculture in Australia | Reinvention, accessed August 5, 2025,

Contextual Support Journal

Regenerative agriculture in Australia: the changing face ... - Frontiers, accessed July 18, 2025

Context

Region Australia
Biome Temperate Grassy Woodlands & Plains
Land Use Agricultural Crop Production
Assessment Conservation Target
Evidence Type ReferenceCondition

Lifecycle

Status Superseded
Version 1
Effective From 11 Mar 2026
Effective To 13 Mar 2026

Notes

Interpretation requires considering microbial biomass carbon and metabolic quotient; a single respiration value alone is ambiguous. The upper detrimental threshold is defined by high qCO2, not respiration rate. The lower critical threshold is related to physical constraints like soil anoxia, not a specific respiration value. No upper detrimental threshold higher values are always better up to natural saturation.

Related Benchmarks

Other benchmarks in the AUS-TGP-AGR-SMR family.