eDNA Biodiversity Detection

AUS-TGP-CON-DNA General High confidence

Benchmark Value

No specific value — see range
Range: 8 to 12 count
Optimal Range: 8 to 12
Direction: Higher is desirable ↑
Form: CompositeFramework

Scoring Curve

Scoring curve unavailable

The scoring engine could not generate a curve for this benchmark context. The primary form is CompositeFramework, but the benchmark data may be missing required fields (e.g., optimal range bounds for an OptimalRange benchmark). This is typically a data quality issue in the benchmark pipeline.

Evidence & Context

The reference value representing the best available condition for a conservation area in the Australian Temperate Grassy Woodlands & Plains biome is defined as: A detected native vertebrate richness of 8-12 species from a single-season aquatic eDNA metabarcoding survey, which must include the presence of at least one EPBC-listed threatened species characteristic of the ecosystem (e.g., Murray Cod, Macquarie Perch, Southern Bell Frog), and a low proportion of invasive species (e.g., invasive species comprise less than 25% of the total number of species detected).

Metric Definition:

eDNA-based vertebrate biodiversity detection compositional benchmark including native species richness, presence of threatened species, and invasive species proportion.

Benchmark Definition:

This benchmark defines the expected native vertebrate species richness and conservation status for aquatic eDNA surveys in conservation areas of the Australian Temperate Grassy Woodlands & Plains biome, requiring 8-12 native species including at least one threatened species and a low invasive species proportion.

Justification:

Derived from the Great Australian Wildlife Search (GAWS) program data, specifically from the Casuarina Sands (ACT) reference site, representing a high-quality, managed conservation area in the target biome.

Sources (3)

Preview of A field ecologist's guide to environmental DNA sampling in freshwater environments - Allen Press, accessed August 12, 2025
A field ecologist's guide to environmental DNA sampling in freshwater environments - Allen Press, accessed August 12, 2025 Journal

What lies beneath the water? | Murray–Darling Basin Authority

View Source
Preview of Biodiversity Research and Monitoring Program 2022-2024 - Report on Implementation - ACT Government, accessed August 12, 2025
Biodiversity Research and Monitoring Program 2022-2024 - Report on Implementation - ACT Government, accessed August 12, 2025

Joint media release: eDNA results reveal rich biodiversity in the Murray–Darling Basin

View Source
Preview of Joint media release: eDNA results reveal rich biodiversity in the ..., accessed August 10, 2025
Joint media release: eDNA results reveal rich biodiversity in the ..., accessed August 10, 2025 Government

Murray–Darling Basin Authority. (2024). eDNA results reveal rich biodiversity in the Murray–Darling Basin. Joint Media Release.

View Source

Supporting Sources (29)

Additional references from the underlying research that informed this benchmark.

Preview of Accumulation curves of environmental DNA sequences predict coastal fish diversity in the coral triangle | Proceedings of the Royal Society B: Biological Sciences - Journals, accessed August 12, 2025
Accumulation curves of environmental DNA sequences predict coastal fish diversity in the coral triangle | Proceedings of the Royal Society B: Biological Sciences - Journals, accessed August 12, 2025
Contextual Support Journal

The National Biodiversity DNA Library – Environomics Future Science Platform, accessed July 23, 2025

View Source
Preview of Ancient and modern environmental DNA | Philosophical Transactions of the Royal Society B, accessed August 12, 2025
Ancient and modern environmental DNA | Philosophical Transactions of the Royal Society B, accessed August 12, 2025
Contextual Support Journal

Ancient and modern environmental DNA | Philosophical Transactions of the Royal Society B, accessed August 12, 2025

View Source
Preview of Basin Condition Monitoring Program, accessed August 12, 2025
Basin Condition Monitoring Program, accessed August 12, 2025
Contextual Support Government

Basin Condition Monitoring Program, accessed August 12, 2025

View Source
Preview of Categories - environmental DNA - CSIRO Data Access Portal, accessed August 12, 2025
Categories - environmental DNA - CSIRO Data Access Portal, accessed August 12, 2025
Contextual Support Government

Categories - environmental DNA - CSIRO Data Access Portal, accessed August 17, 2025

View Source
Preview of eDNA explained: unlocking nature's hidden biodiversity - CSIRO, accessed August 12, 2025
eDNA explained: unlocking nature's hidden biodiversity - CSIRO, accessed August 12, 2025
Methodology Source Government

www.csiro.au

View Source
Preview of eDNA results from the Great Australian Wildlife Search | Murray–Darling Basin Authority, accessed August 10, 2025
eDNA results from the Great Australian Wildlife Search | Murray–Darling Basin Authority, accessed August 10, 2025
Direct Evidence Journal

Murray–Darling Basin Authority. (2024). EDNA Results From The Great Australian Wildlife Search.

View Source
Preview of eDNA shows diverse wildlife in the rivers of the Murray–Darling Basin, accessed August 10, 2025
eDNA shows diverse wildlife in the rivers of the Murray–Darling Basin, accessed August 10, 2025
Direct Evidence

eDNA results from the Great Australian Wildlife Search | Murray–Darling Basin Authority, accessed August 10, 2025

View Source
Preview of eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed August 12, 2025
eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed August 12, 2025
Contextual Support Journal

Correlated environmental DNA and electrofished estimates of relative biomass for golden perch, Macquaria ambigua - Frontiers, accessed July 20, 2025

View Source
Preview of Effects of Stock Grazing on Biodiversity Values in ... - ACT Government, accessed August 4, 2025
Effects of Stock Grazing on Biodiversity Values in ... - ACT Government, accessed August 4, 2025
Contextual Support Journal

Yellow Box – Blakely's Red Gum Grassy Woodland and Derived ...

View Source
Preview of EnviroDNA: eDNA Biodiversity Monitoring Solutions, accessed August 12, 2025
EnviroDNA: eDNA Biodiversity Monitoring Solutions, accessed August 12, 2025
Methodology Source GreyLiterature

EnviroDNA: eDNA Biodiversity Monitoring Solutions

View Source
Preview of Environmental DNA (eDNA) - U.S. Fish and Wildlife Service, accessed August 12, 2025
Environmental DNA (eDNA) - U.S. Fish and Wildlife Service, accessed August 12, 2025
Contextual Support Journal

Joint media release: eDNA results reveal rich biodiversity in the Murray–Darling Basin

View Source
Preview of Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review - Oxford Academic, accessed August 12, 2025
Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review - Oxford Academic, accessed August 12, 2025
Contextual Support Journal

Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review - Oxford Academic, accessed August 12, 2025

View Source
Preview of Environmental projects - Murray–Darling Basin Authority, accessed August 12, 2025
Environmental projects - Murray–Darling Basin Authority, accessed August 12, 2025
Contextual Support Government

Environmental projects - Murray–Darling Basin Authority, accessed August 12, 2025

View Source
Preview of Evaluating eDNA and metabarcoding as biodiversity monitoring tools, accessed August 12, 2025
Evaluating eDNA and metabarcoding as biodiversity monitoring tools, accessed August 12, 2025
Methodology Source Government

eDNA methods for tropical waters - Northern Australia - Resilient Landscapes Hub, accessed July 20, 2025

View Source
Preview of Great Australian Wildlife Search, accessed August 12, 2025
Great Australian Wildlife Search, accessed August 12, 2025
Methodology Source GreyLiterature

Great Australian Wildlife Search, accessed August 12, 2025

View Source
Preview of Join the Great Australian Wildlife Search! | Chuffed, accessed August 12, 2025
Join the Great Australian Wildlife Search! | Chuffed, accessed August 12, 2025
Contextual Support GreyLiterature

Join the Great Australian Wildlife Search! | Chuffed, accessed August 12, 2025

View Source
Preview of Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient, accessed August 12, 2025
Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient, accessed August 12, 2025
Methodology Source Journal

Comparing morphological and DNA-based bioassessment methodologies for macroinvertebrates in Neotropical streams: a case study from Ecuador - Metabarcoding and Metagenomics, accessed August 17, 2025,

View Source
Preview of More (or less?) bounce for the ounce: a comparison of environmental DNA and classical approaches for bioassessment - CSIRO PUBLISHING | Marine and Freshwater Research, accessed August 12, 2025
More (or less?) bounce for the ounce: a comparison of environmental DNA and classical approaches for bioassessment - CSIRO PUBLISHING | Marine and Freshwater Research, accessed August 12, 2025
Methodology Source Journal

More (or less?) bounce for the ounce: a comparison of environmental DNA and classical approaches for bioassessment - CSIRO PUBLISHING | Marine and Freshwater Research, accessed August 12, 2025

View Source
Preview of NatureMetrics Raises Funding with Environmental DNA Tech - Taskforce on Nature-related Financial Disclosures (TNFD), accessed August 12, 2025
NatureMetrics Raises Funding with Environmental DNA Tech - Taskforce on Nature-related Financial Disclosures (TNFD), accessed August 12, 2025
Contextual Support Journal

4 ways eDNA reduces invasive species risk - Nature Metrics

View Source
Preview of Recent initiatives in biodiversity conservation in grazed temperate grasslands and woodlands in Australia - UKnowledge, accessed August 12, 2025,
Recent initiatives in biodiversity conservation in grazed temperate grasslands and woodlands in Australia - UKnowledge, accessed August 12, 2025,
Contextual Support

Processes underpinning natural capital account compilation highlight the potential for low-input grazing to mitigate farm carbon emissions while also improving biodiversity outcomes - CSIRO PUBLISHING | The Rangeland Journal, accessed August 12, 2025

View Source
Preview of Revolutionising environmental monitoring using eDNA - CSIRO, accessed August 12, 2025
Revolutionising environmental monitoring using eDNA - CSIRO, accessed August 12, 2025
Methodology Source Government

Environmental DNA (eDNA) - U.S. Fish and Wildlife Service

View Source
Preview of South eastern Australia - POLICYMIX, accessed August 12, 2025
South eastern Australia - POLICYMIX, accessed August 12, 2025
Contextual Support GreyLiterature

South eastern Australia - POLICYMIX, accessed August 12, 2025

View Source
Preview of SUSTAINABLE FARMS - Annual Report 2023, accessed August 12, 2025
SUSTAINABLE FARMS - Annual Report 2023, accessed August 12, 2025
Contextual Support Journal

SUSTAINABLE FARMS - Annual Report 2023, accessed August 12, 2025

View Source
Preview of The Great Australian Wildlife Search announces public release of eDNA data across Australia's largest river system - EnviroDNA, accessed August 12, 2025
The Great Australian Wildlife Search announces public release of eDNA data across Australia's largest river system - EnviroDNA, accessed August 12, 2025
Contextual Support GreyLiterature

A Pilot Study on the Utility of eDNA Monitoring for Inland Australian Waterbirds - Macquarie University

View Source
Preview of The Great Australian Wildlife Search Large-scale biodiversity assessments using eDNA metabarcoding and citizen scientists, accessed August 12, 2025
The Great Australian Wildlife Search Large-scale biodiversity assessments using eDNA metabarcoding and citizen scientists, accessed August 12, 2025
Direct Evidence Journal

Murray–Darling Basin Spring 2024 Biodiversity Findings Report, accessed July 20, 2025

View Source
Preview of The Great Australian Wildlife Search: Large-scale biodiversity assessments using eDNA metabarcoding and citizen scientists., accessed August 12, 2025
The Great Australian Wildlife Search: Large-scale biodiversity assessments using eDNA metabarcoding and citizen scientists., accessed August 12, 2025
Methodology Source GreyLiterature

What lies beneath the water? | Murray–Darling Basin Authority, accessed July 19, 2025

View Source
Preview of The value of a National Biodiversity DNA Library - CSIRO Research, accessed August 12, 2025
The value of a National Biodiversity DNA Library - CSIRO Research, accessed August 12, 2025
Contextual Support Journal

Environmental DNA metabarcoding for marine monitoring across ecological scales - the UWA Profiles and Research Repository - The University of Western Australia

View Source
Preview of Toward an ecoregion scale evaluation of eDNA metabarcoding ..., accessed August 10, 2025
Toward an ecoregion scale evaluation of eDNA metabarcoding ..., accessed August 10, 2025
Methodology Source Journal

Murray–Darling Basin Spring 2024 Biodiversity Findings Report, accessed July 19, 2025

View Source
Preview of What lies beneath the water? | Murray–Darling Basin Authority, accessed August 12, 2025
What lies beneath the water? | Murray–Darling Basin Authority, accessed August 12, 2025
Contextual Support Government

Accounting for Nature and measuring nature repair - Greening ...

View Source

Context

  • Region Australia
  • Biome Temperate Grassy Woodlands & Plains
  • Land Use Conservation / Protected Natural Areas
  • Assessment Conservation Target
  • Evidence Type ReferenceCondition

Lifecycle

  • Status Active
  • Version 1
  • Effective From 3 Jun 2026

Notes

The benchmark is compositional, requiring not just a number of native species but also the presence of high-conservation-value threatened species and a low ratio (<25%) of invasive to native species, reflecting true ecological health.

Related Benchmarks

Other benchmarks in the AUS-TGP-CON-DNA family.