eDNA Biodiversity Detection

AUS-ASC-AQU-DNA General Moderate confidence

Benchmark Value

93 count

Direction: Higher is desirable ↑

Form: MinimumOnly

Scoring Curve

Scoring curve unavailable

The scoring engine could not generate a curve for this benchmark context. The primary form is CompositeFramework, but the benchmark data may be missing required fields (e.g., optimal range bounds for an OptimalRange benchmark). This is typically a data quality issue in the benchmark pipeline.

Contributing Benchmarks

CompositeFramework Primary OptimalRange Guard Point Guard

Evidence & Context

The combined 16S and 18S metabarcoding method was found to be cheaper than the traditional morphology-based method when analyzing a large number of samples (approximately 93-100 or more)

Metric Definition:

The sample size threshold at which eDNA metabarcoding (16S and 18S) becomes more cost-effective than traditional morphology-based surveys for monitoring aquaculture enriched soft sediments.

Benchmark Definition:

This benchmark defines the minimum sample size at which eDNA metabarcoding becomes more cost-effective than traditional morphology-based surveys in aquaculture environments within the Alpine and Subalpine Complex biome.

Justification:

Based on a comparative study of Atlantic Salmon farms in Tasmania assessing benthic health.

Sources (1)

Preview of Comparing eDNA metabarcoding and morphology-based surveys for environmental monitoring of aquaculture enriched soft sediments., accessed August 17, 2025

Comparing eDNA metabarcoding and morphology-based surveys for environmental monitoring of aquaculture enriched soft sediments., accessed August 17, 2025 Journal

Comparing eDNA metabarcoding and morphology-based surveys for environmental monitoring of aquaculture enriched soft sediments., accessed August 17, 2025

Supporting Sources (47)

Additional references from the underlying research that informed this benchmark.

Preview of (PDF) Climate change impacts on Australia's alpine ecosystems - ResearchGate, accessed on May 28, 2025,

(PDF) Climate change impacts on Australia's alpine ecosystems - ResearchGate, accessed on May 28, 2025,

Contextual Support GreyLiterature

(PDF) Climate change impacts on Australia's alpine ecosystems - ResearchGate, accessed August 5, 2025

Preview of A comparison of visual and molecular methods for inferring ..., accessed August 17, 2025

A comparison of visual and molecular methods for inferring ..., accessed August 17, 2025

Methodology Source GreyLiterature

A comparison of visual and molecular methods for inferring ..., accessed August 17, 2025

Preview of A field ecologist's guide to environmental DNA sampling in freshwater environments - Allen Press, accessed August 12, 2025

A field ecologist's guide to environmental DNA sampling in freshwater environments - Allen Press, accessed August 12, 2025

Methodology Source Journal

What lies beneath the water? | Murray–Darling Basin Authority

Preview of A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository

A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository

Contextual Support Journal

TICI: a taxon-independent community index for eDNA-based ecological health assessment

Preview of A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository, accessed August 17, 2025

A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository, accessed August 17, 2025

Methodology Source Government

A three-year, Australian-wide, environmental DNA (eDNA) study reveals spatial and temporal patterns in marine biodiversity - CSIRO Research Publications Repository, accessed August 17, 2025

Preview of An issue of life or death: using eDNA to detect viable individuals in wilderness restoration, accessed August 4, 2025

An issue of life or death: using eDNA to detect viable individuals in wilderness restoration, accessed August 4, 2025

Contextual Support GreyLiterature

An issue of life or death: using eDNA to detect viable individuals in wilderness restoration, accessed August 4, 2025,

Preview of Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution, accessed August 17, 2025

Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution, accessed August 17, 2025

Contextual Support Journal

Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution, accessed August 17, 2025

Preview of Assessment of the Values of Kosciuszko National Park (Chapter 8), accessed August 17, 2025,

Assessment of the Values of Kosciuszko National Park (Chapter 8), accessed August 17, 2025,

Contextual Support Journal

eDNA Is a Useful Environmental Monitoring Tool for Assessing Stream Ecological Health, accessed August 17, 2025

Preview of Australian Mammalogy - CSIRO PUBLISHING

Australian Mammalogy - CSIRO PUBLISHING

Methodology Source Journal

Australian Mammalogy - CSIRO PUBLISHING, accessed August 17, 2025

Preview of Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis - Frontiers, accessed August 10, 2025

Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis - Frontiers, accessed August 10, 2025

Contextual Support Journal

Beyond Detection – The Future of eDNA Research (Post-Event Summary)

Preview of Categories - environmental DNA - CSIRO Data Access Portal, accessed August 12, 2025

Categories - environmental DNA - CSIRO Data Access Portal, accessed August 12, 2025

Contextual Support Government

Categories - environmental DNA - CSIRO Data Access Portal, accessed August 17, 2025

Preview of Correlated environmental DNA and electrofished estimates of relative biomass for golden perch, Macquaria ambigua - Frontiers, accessed August 17, 2025

Correlated environmental DNA and electrofished estimates of relative biomass for golden perch, Macquaria ambigua - Frontiers, accessed August 17, 2025

Methodology Source Journal

Correlated environmental DNA and electrofished estimates of relative biomass for golden perch, Macquaria ambigua - Frontiers, accessed August 17, 2025

Preview of eDNA - an innovative survey method, accessed August 17, 2025

eDNA - an innovative survey method, accessed August 17, 2025

Contextual Support GreyLiterature

eDNA - an innovative survey method, accessed August 17, 2025

Preview of eDNA as a tool for identifying freshwater species in sustainable forestry - Forest Service, accessed July 21, 2025,

eDNA as a tool for identifying freshwater species in sustainable forestry - Forest Service, accessed July 21, 2025,

Contextual Support Journal

Catchment-based sampling of river eDNA integrates terrestrial and ..., accessed August 17, 2025

Preview of eDNA Is a Useful Environmental Monitoring Tool for Assessing Stream Ecological Health

eDNA Is a Useful Environmental Monitoring Tool for Assessing Stream Ecological Health

Contextual Support Journal

Centre Spotlight: Research Centre for Applied Alpine Ecology ..., accessed August 17, 2025

Preview of eDNA methods for tropical waters - Northern Australia - Resilient Landscapes Hub, accessed July 11, 2025,

eDNA methods for tropical waters - Northern Australia - Resilient Landscapes Hub, accessed July 11, 2025,

Methodology Source GreyLiterature

Environmental DNA (eDNA) in the ALA - Atlas of Living Australia

Preview of eDNA results from the Great Australian Wildlife Search | Murray–Darling Basin Authority, accessed August 10, 2025

eDNA results from the Great Australian Wildlife Search | Murray–Darling Basin Authority, accessed August 10, 2025

Direct Evidence Journal

Murray–Darling Basin Authority. (2024). EDNA Results From The Great Australian Wildlife Search.

Preview of eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed August 12, 2025

eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed August 12, 2025

Methodology Source Journal

Correlated environmental DNA and electrofished estimates of relative biomass for golden perch, Macquaria ambigua - Frontiers, accessed July 20, 2025

Preview of eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed July 22, 2025,

eDNA: Review of applicability for monitoring and detecting biotic populations of the Murray-Darling Basin - Centre for Invasive Species Solutions, accessed July 22, 2025,

Methodology Source Government

Integrating environmental DNA science into Australia's marine parks: a roadmap - CSIRO Research Publications Repository

Preview of EnviroDNA: eDNA Biodiversity Monitoring Solutions, accessed August 12, 2025

EnviroDNA: eDNA Biodiversity Monitoring Solutions, accessed August 12, 2025

Methodology Source GreyLiterature

EnviroDNA: eDNA Biodiversity Monitoring Solutions

Preview of Environmental DNA (eDNA) in the ALA - Atlas of Living Australia

Environmental DNA (eDNA) in the ALA - Atlas of Living Australia

Contextual Support Journal

Bridging the gap: The integration of eDNA techniques and traditional sampling in fish diversity analysis - Frontiers, accessed August 17, 2025

Preview of Environmental DNA (eDNA) technology: Fisheries and aquaculture perspectives - IJAH, accessed August 10, 2025, )-Rev.pdf

Environmental DNA (eDNA) technology: Fisheries and aquaculture perspectives - IJAH, accessed August 10, 2025, )-Rev.pdf

Direct Evidence

Environmental DNA (eDNA) technology: Fisheries and aquaculture perspectives - IJAH

Preview of Environmental DNA (eDNA) technology: Fisheries and aquaculture perspectives - Indian Journal of Animal Health, accessed July 13, 2025, )-Rev.pdf

Environmental DNA (eDNA) technology: Fisheries and aquaculture perspectives - Indian Journal of Animal Health, accessed July 13, 2025, )-Rev.pdf

Contextual Support GreyLiterature

Evaluating a multigene environmental DNA approach for biodiversity assessment - PMC, accessed August 17, 2025

Preview of Environmental DNA dispersal from Atlantic salmon farms - Canadian Science Publishing, accessed August 17, 2025

Environmental DNA dispersal from Atlantic salmon farms - Canadian Science Publishing, accessed August 17, 2025

Contextual Support Journal

Environmental DNA dispersal from Atlantic salmon farms - Canadian Science Publishing, accessed August 17, 2025

Preview of Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks - PMC, accessed August 17, 2025

Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks - PMC, accessed August 17, 2025

Methodology Source

Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks - PMC, accessed August 17, 2025

Preview of Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting - REABIC, accessed August 17, 2025

Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting - REABIC, accessed August 17, 2025

Direct Evidence Journal

Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting - REABIC, accessed August 17, 2025

Preview of Environmental DNA simultaneously informs hydrological and biodiversity characterization of an Alpine catchment - HESS

Environmental DNA simultaneously informs hydrological and biodiversity characterization of an Alpine catchment - HESS

Contextual Support Journal

Environmental DNA simultaneously informs hydrological and biodiversity characterization of an Alpine catchment - HESS, accessed August 17, 2025

Preview of Environmental DNA: A New Low-Cost Monitoring Tool for Pathogens in Salmonid Aquaculture - Frontiers, accessed August 17, 2025

Environmental DNA: A New Low-Cost Monitoring Tool for Pathogens in Salmonid Aquaculture - Frontiers, accessed August 17, 2025

Direct Evidence

Environmental DNA: A New Low-Cost Monitoring Tool for Pathogens in Salmonid Aquaculture - Frontiers, accessed August 17, 2025

Preview of Estimating species richness using environmental DNA - PMC - PubMed Central, accessed August 10, 2025

Estimating species richness using environmental DNA - PMC - PubMed Central, accessed August 10, 2025

Contextual Support Journal

Estuarine fishes associated with intertidal oyster reefs characterized using environmental DNA and baited remote underwater video

Preview of Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems - PubMed

Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems - PubMed

Contextual Support Journal

SEA-MES coupled eDNA experiment – Southeast Australian Marine Ecosystem Survey, accessed August 17, 2025

Preview of Fish community assessment with eDNA metabarcoding: effects of sampling design and bioinformatic filtering - Canadian Science Publishing, accessed August 10, 2025

Fish community assessment with eDNA metabarcoding: effects of sampling design and bioinformatic filtering - Canadian Science Publishing, accessed August 10, 2025

Contextual Support Journal

Fish community assessment with eDNA metabarcoding: effects of sampling design and bioinformatic filtering - Canadian Science Publishing, accessed August 17, 2025

Preview of Guidance for environmental DNA sampling design and effort - Great Lakes Fishery Commission, accessed August 17, 2025

Guidance for environmental DNA sampling design and effort - Great Lakes Fishery Commission, accessed August 17, 2025

Contextual Support GreyLiterature

Guidance for environmental DNA sampling design and effort - Great Lakes Fishery Commission, accessed August 17, 2025

Preview of Integrating environmental DNA science into Australia's marine parks: a roadmap - CSIRO

Integrating environmental DNA science into Australia's marine parks: a roadmap - CSIRO

Direct Evidence Journal

Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution

Preview of Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach

Invasive terrestrial invertebrate detection in water and soil using a targeted eDNA approach

Direct Evidence

Australia's forests - Australian forest profiles, accessed July 24, 2025,

Preview of Joint media release: eDNA results reveal rich biodiversity in the ..., accessed August 10, 2025

Joint media release: eDNA results reveal rich biodiversity in the ..., accessed August 10, 2025

Methodology Source Government

Murray–Darling Basin Authority. (2024). eDNA results reveal rich biodiversity in the Murray–Darling Basin. Joint Media Release.

Preview of New environmental DNA (eDNA) data a game-changer for understanding life in our oceans, accessed August 4, 2025

New environmental DNA (eDNA) data a game-changer for understanding life in our oceans, accessed August 4, 2025

Contextual Support Journal

Unveiling the Nation's Bold Strategy to Advance Environmental DNA: Key Workshop Takeaways

Preview of Real time eDNA tools to improve early detection and response ..., accessed August 17, 2025

Real time eDNA tools to improve early detection and response ..., accessed August 17, 2025

Contextual Support GreyLiterature

Real time eDNA tools to improve early detection and response ..., accessed August 17, 2025

Preview of Report Interpretation Guide - Nature Metrics, accessed August 17, 2025

Report Interpretation Guide - Nature Metrics, accessed August 17, 2025

Methodology Source

Report Interpretation Guide - Nature Metrics, accessed August 17, 2025

Preview of Soil microbiome and biodiversity implications – are we ignoring cost ...

Soil microbiome and biodiversity implications – are we ignoring cost ...

Contextual Support Journal

Monitoring riverine fish communities through eDNA metabarcoding ..., accessed August 17, 2025

Preview of Soil microbiome and biodiversity implications – are we ignoring cost-effective proxies of biodiversity measures? | TERN Australia

Soil microbiome and biodiversity implications – are we ignoring cost-effective proxies of biodiversity measures? | TERN Australia

Direct Evidence GreyLiterature

Wang, S., et al. (2022). Adaptive multi-paddock grazing increases soil nutrient availability and bacteria to fungi ratio in grassland soils. Applied Soil Ecology, 179, 104590.

Preview of Tassie Salomon farming. What's the story behind it? : r/tasmania - Reddit, accessed August 17, 2025

Tassie Salomon farming. What's the story behind it? : r/tasmania - Reddit, accessed August 17, 2025

Contextual Support GreyLiterature

Tassie Salomon farming. What's the story behind it? : r/tasmania - Reddit, accessed August 17, 2025

Preview of The application of environmental DNA (eDNA) methods for the monitoring and detection of aquatic microorganisms in aquaculture - BORIS Theses, accessed August 17, 2025

The application of environmental DNA (eDNA) methods for the monitoring and detection of aquatic microorganisms in aquaculture - BORIS Theses, accessed August 17, 2025

Contextual Support Journal

The application of environmental DNA (eDNA) methods for the monitoring and detection of aquatic microorganisms in aquaculture - BORIS Theses, accessed August 17, 2025

Preview of The Promise and Pitfalls of Environmental DNA and RNA Approaches for the Monitoring of Human and Animal Pathogens from Aquatic Sources, accessed August 17, 2025

The Promise and Pitfalls of Environmental DNA and RNA Approaches for the Monitoring of Human and Animal Pathogens from Aquatic Sources, accessed August 17, 2025

Contextual Support Journal

The Promise and Pitfalls of Environmental DNA and RNA Approaches for the Monitoring of Human and Animal Pathogens from Aquatic Sources, accessed August 17, 2025

Preview of The Viability and Potential of Environmental DNA (eDNA) Detection of Freshwater Fish Based on Current Genetic Resources in Malay - Universiti Kebangsaan Malaysia, accessed August 17, 2025

The Viability and Potential of Environmental DNA (eDNA) Detection of Freshwater Fish Based on Current Genetic Resources in Malay - Universiti Kebangsaan Malaysia, accessed August 17, 2025

Methodology Source Journal

The Viability and Potential of Environmental DNA (eDNA) Detection of Freshwater Fish Based on Current Genetic Resources in Malay - Universiti Kebangsaan Malaysia, accessed August 17, 2025

Preview of Toward an ecoregion scale evaluation of eDNA metabarcoding primers: A case study for the freshwater fish biodiversity of the Murray–Darling Basin (Australia) - CSIRO Research Publications Repository, accessed August 17, 2025

Toward an ecoregion scale evaluation of eDNA metabarcoding primers: A case study for the freshwater fish biodiversity of the Murray–Darling Basin (Australia) - CSIRO Research Publications Repository, accessed August 17, 2025

Contextual Support GreyLiterature

Toward an ecoregion scale evaluation of eDNA metabarcoding primers: A case study for the freshwater fish biodiversity of the Murray–Darling Basin (Australia) - CSIRO Research Publications Repository, accessed August 17, 2025

Preview of Use of eDNA and conventional sampling methods to survey rock pool (gnamma) biodiversity on granite inselbergs, accessed August 17, 2025

Use of eDNA and conventional sampling methods to survey rock pool (gnamma) biodiversity on granite inselbergs, accessed August 17, 2025

Methodology Source GreyLiterature

Use of eDNA and conventional sampling methods to survey rock pool (gnamma) biodiversity on granite inselbergs, accessed August 17, 2025

Preview of What lies beneath the water? | Murray–Darling Basin Authority

What lies beneath the water? | Murray–Darling Basin Authority

Contextual Support GreyLiterature

eDNA: A NEW TOOL TO ESTABLISH INVASIVE SPECIES ERADICATION SUCCESS, accessed August 17, 2025

Context

Region Australia
Biome Alpine and Subalpine Complex
Land Use Aquaculture
Assessment Not Stated
Evidence Type ReferenceCondition

Lifecycle

Status Superseded
Version 3
Effective From 27 May 2026
Effective To 27 May 2026

Notes

The document specifies a range of 93-100 samples for cost-effectiveness. AssessmentContext defaulted to 'Not Stated' because the source document did not state one.

Related Benchmarks

Other benchmarks in the AUS-ASC-AQU-DNA family.